Review





Similar Products

anti human plcγ1 antibody  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti human plcγ1 antibody
Anti Human Plcγ1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human plcγ1 antibody/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
anti human plcγ1 antibody - by Bioz Stars, 2026-06
95/100 stars
  Buy from Supplier
p plcγ1 tyr783  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc p plcγ1 tyr783
P Plcγ1 Tyr783, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p plcγ1 tyr783/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
p plcγ1 tyr783 - by Bioz Stars, 2026-06
96/100 stars
  Buy from Supplier
antibodies targeting plcγ1  (Affinity Biosciences)
86
Affinity Biosciences antibodies targeting plcγ1
TQHS increased <t>PLCγ1</t> expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.
Antibodies Targeting Plcγ1, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies targeting plcγ1/product/Affinity Biosciences
Average 86 stars, based on 1 article reviews
antibodies targeting plcγ1 - by Bioz Stars, 2026-06
86/100 stars
  Buy from Supplier
antibodies against p plcγ1  (Affinity Biosciences)
86
Affinity Biosciences antibodies against p plcγ1
TQHS increased <t>PLCγ1</t> expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.
Antibodies Against P Plcγ1, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against p plcγ1/product/Affinity Biosciences
Average 86 stars, based on 1 article reviews
antibodies against p plcγ1 - by Bioz Stars, 2026-06
86/100 stars
  Buy from Supplier
anti phospho plcγ1 tyr783 pplcγ  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti phospho plcγ1 tyr783 pplcγ
TQHS increased <t>PLCγ1</t> expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.
Anti Phospho Plcγ1 Tyr783 Pplcγ, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phospho plcγ1 tyr783 pplcγ/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
anti phospho plcγ1 tyr783 pplcγ - by Bioz Stars, 2026-06
96/100 stars
  Buy from Supplier
phospho plcγ1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phospho plcγ1
TQHS increased <t>PLCγ1</t> expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.
Phospho Plcγ1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho plcγ1/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
phospho plcγ1 - by Bioz Stars, 2026-06
96/100 stars
  Buy from Supplier
plcγ1 protein  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc plcγ1 protein
TQHS increased <t>PLCγ1</t> expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.
Plcγ1 Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plcγ1 protein/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
plcγ1 protein - by Bioz Stars, 2026-06
95/100 stars
  Buy from Supplier
plcγ1 tyr 783  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc plcγ1 tyr 783
TQHS increased <t>PLCγ1</t> expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.
Plcγ1 Tyr 783, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plcγ1 tyr 783/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
plcγ1 tyr 783 - by Bioz Stars, 2026-06
96/100 stars
  Buy from Supplier
9101s anti phospho plcγ1 tyr783 cell signaling technology  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc 9101s anti phospho plcγ1 tyr783 cell signaling technology
TQHS increased <t>PLCγ1</t> expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.
9101s Anti Phospho Plcγ1 Tyr783 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/9101s anti phospho plcγ1 tyr783 cell signaling technology/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
9101s anti phospho plcγ1 tyr783 cell signaling technology - by Bioz Stars, 2026-06
99/100 stars
  Buy from Supplier
anti phospho plcγ1 tyr783  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti phospho plcγ1 tyr783
TQHS increased <t>PLCγ1</t> expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.
Anti Phospho Plcγ1 Tyr783, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phospho plcγ1 tyr783/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
anti phospho plcγ1 tyr783 - by Bioz Stars, 2026-06
96/100 stars
  Buy from Supplier

Image Search Results


TQHS increased PLCγ1 expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.

Journal: Frontiers in Aging Neuroscience

Article Title: Exploring the neuroprotective mechanism of Tongqiao Huashuan granules in vascular dementia based on PLCγ1/IP3R signaling pathway

doi: 10.3389/fnagi.2026.1719664

Figure Lengend Snippet: TQHS increased PLCγ1 expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.

Article Snippet: Brain coronal sections were blocked in 10% goat serum (C0265, Beyotime Biotech Inc., China) at ambient temperature for 1 h, subsequently incubated overnight at 4°C with primary antibodies targeting PLCγ1 (1:200 dilution, AF6210, AB_2835091, Affinity Biosciences, United States) and CaM (1:200 dilution, AF6353, AB_2835158, Affinity Biosciences, United States).

Techniques: Expressing, Immunohistochemical staining

TQHS increased PLCγ1, p-PLCγ1, IP3R and CAMKKII expression, whereas reduced CaM expression in the hippocampus of VaD rats. (A,F) Western blot was performed to detect the levels of PLCγ1, p-PLCγ1, IP3R, CAMKKII and CaM in the hippocampus of VaD rats. Quantitative analysis of (B) PLCγ1, (C) CAMKKII, (D) p-PLCγ1, (E) IP3R and (G) CaM expression. The densities of the bands were normalized with respect to the values of GADPH and β-actin. Values were expressed as means ± SD ( n = 3). ### P < 0.001 versus sham-surgery group. ** P < 0.01, *** P < 0.001 versus model group.

Journal: Frontiers in Aging Neuroscience

Article Title: Exploring the neuroprotective mechanism of Tongqiao Huashuan granules in vascular dementia based on PLCγ1/IP3R signaling pathway

doi: 10.3389/fnagi.2026.1719664

Figure Lengend Snippet: TQHS increased PLCγ1, p-PLCγ1, IP3R and CAMKKII expression, whereas reduced CaM expression in the hippocampus of VaD rats. (A,F) Western blot was performed to detect the levels of PLCγ1, p-PLCγ1, IP3R, CAMKKII and CaM in the hippocampus of VaD rats. Quantitative analysis of (B) PLCγ1, (C) CAMKKII, (D) p-PLCγ1, (E) IP3R and (G) CaM expression. The densities of the bands were normalized with respect to the values of GADPH and β-actin. Values were expressed as means ± SD ( n = 3). ### P < 0.001 versus sham-surgery group. ** P < 0.01, *** P < 0.001 versus model group.

Article Snippet: Brain coronal sections were blocked in 10% goat serum (C0265, Beyotime Biotech Inc., China) at ambient temperature for 1 h, subsequently incubated overnight at 4°C with primary antibodies targeting PLCγ1 (1:200 dilution, AF6210, AB_2835091, Affinity Biosciences, United States) and CaM (1:200 dilution, AF6353, AB_2835158, Affinity Biosciences, United States).

Techniques: Expressing, Western Blot

Effects of TQHS granules on OGD/R-induced cytotoxicity SH-SY5Y cells and the mRNA expressions of PLCγ1, IP3R, and TrkB. (A) Effects of U73122 on normal SH-SY5Y cell viability. (B,C) Effects of B-U73122 and (C) TQHS granules on OGD/G cell viability. (D–F) RT-qPCR analyzed (D) TrkB, (E) IP3R and (F) PLCγ1 mRNA expression levels. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus control group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus OGD/R group.

Journal: Frontiers in Aging Neuroscience

Article Title: Exploring the neuroprotective mechanism of Tongqiao Huashuan granules in vascular dementia based on PLCγ1/IP3R signaling pathway

doi: 10.3389/fnagi.2026.1719664

Figure Lengend Snippet: Effects of TQHS granules on OGD/R-induced cytotoxicity SH-SY5Y cells and the mRNA expressions of PLCγ1, IP3R, and TrkB. (A) Effects of U73122 on normal SH-SY5Y cell viability. (B,C) Effects of B-U73122 and (C) TQHS granules on OGD/G cell viability. (D–F) RT-qPCR analyzed (D) TrkB, (E) IP3R and (F) PLCγ1 mRNA expression levels. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus control group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus OGD/R group.

Article Snippet: Brain coronal sections were blocked in 10% goat serum (C0265, Beyotime Biotech Inc., China) at ambient temperature for 1 h, subsequently incubated overnight at 4°C with primary antibodies targeting PLCγ1 (1:200 dilution, AF6210, AB_2835091, Affinity Biosciences, United States) and CaM (1:200 dilution, AF6353, AB_2835158, Affinity Biosciences, United States).

Techniques: Quantitative RT-PCR, Expressing, Control

Potential mechanism underlying the neuroprotective effects of TQHS granules in VaD rats and SH-SY5Y cells. TQHS granules improved cognitive function in VaD rats, generated IP3 through PLCγ1-mediated catalytic hydrolysis of PIP2, and then affected the expression of IP3R downstream of the pathway, thus protecting the brain from VaD-induced oxidative stress and calcium overload, and further induced antioxidant activity and regulated intracellular calcium overload mechanisms.

Journal: Frontiers in Aging Neuroscience

Article Title: Exploring the neuroprotective mechanism of Tongqiao Huashuan granules in vascular dementia based on PLCγ1/IP3R signaling pathway

doi: 10.3389/fnagi.2026.1719664

Figure Lengend Snippet: Potential mechanism underlying the neuroprotective effects of TQHS granules in VaD rats and SH-SY5Y cells. TQHS granules improved cognitive function in VaD rats, generated IP3 through PLCγ1-mediated catalytic hydrolysis of PIP2, and then affected the expression of IP3R downstream of the pathway, thus protecting the brain from VaD-induced oxidative stress and calcium overload, and further induced antioxidant activity and regulated intracellular calcium overload mechanisms.

Article Snippet: Brain coronal sections were blocked in 10% goat serum (C0265, Beyotime Biotech Inc., China) at ambient temperature for 1 h, subsequently incubated overnight at 4°C with primary antibodies targeting PLCγ1 (1:200 dilution, AF6210, AB_2835091, Affinity Biosciences, United States) and CaM (1:200 dilution, AF6353, AB_2835158, Affinity Biosciences, United States).

Techniques: Generated, Expressing, Antioxidant Activity Assay

TQHS increased PLCγ1 expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.

Journal: Frontiers in Aging Neuroscience

Article Title: Exploring the neuroprotective mechanism of Tongqiao Huashuan granules in vascular dementia based on PLCγ1/IP3R signaling pathway

doi: 10.3389/fnagi.2026.1719664

Figure Lengend Snippet: TQHS increased PLCγ1 expression, whereas reduced CaM expression in the hippocampal CA1 and CA3 regions of VaD Rats. (A) Immunohistochemical analysis of PLCγ1 and CaM expression in hippocampal CA1 and CA3 regions (× 10) ( n = 2). (B–E) Quantitative analysis showed the expression of (B) PLCγ1, and (D) CaM in hippocampal CA1 regions and (C) PLCγ1, and (E) CaM in hippocampal CA3 regions. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus sham-surgery group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus model group.

Article Snippet: Following washes and blocking (5% skim milk, 1 h), membranes were probed overnight (4°C) with primary antibodies against p-PLCγ1 (1:2,000 dilution, AF3210, AB_2834502, Affinity Biosciences, United States), PLCγ1 (1:2,000 dilution, AF6210, AB_2835091, Affinity Biosciences, United States), IP3R (1:2000 dilution, DF3000, AB_2840979, Affinity Biosciences, United States), CAMKKII (1:1,000 dilution, DF4793, AB_2837147, Affinity Biosciences, United States), GAPDH (1:50,000 dilution, 60004-1-Ig, AB_2107436, Proteintech, China), CaM (1:2,000 dilution, AF6353, AB_2835158, Affinity Biosciences, United States) and β-actin (1:10,000 dilution, AF7018, AB_2839420, Affinity Biosciences, United States).

Techniques: Expressing, Immunohistochemical staining

TQHS increased PLCγ1, p-PLCγ1, IP3R and CAMKKII expression, whereas reduced CaM expression in the hippocampus of VaD rats. (A,F) Western blot was performed to detect the levels of PLCγ1, p-PLCγ1, IP3R, CAMKKII and CaM in the hippocampus of VaD rats. Quantitative analysis of (B) PLCγ1, (C) CAMKKII, (D) p-PLCγ1, (E) IP3R and (G) CaM expression. The densities of the bands were normalized with respect to the values of GADPH and β-actin. Values were expressed as means ± SD ( n = 3). ### P < 0.001 versus sham-surgery group. ** P < 0.01, *** P < 0.001 versus model group.

Journal: Frontiers in Aging Neuroscience

Article Title: Exploring the neuroprotective mechanism of Tongqiao Huashuan granules in vascular dementia based on PLCγ1/IP3R signaling pathway

doi: 10.3389/fnagi.2026.1719664

Figure Lengend Snippet: TQHS increased PLCγ1, p-PLCγ1, IP3R and CAMKKII expression, whereas reduced CaM expression in the hippocampus of VaD rats. (A,F) Western blot was performed to detect the levels of PLCγ1, p-PLCγ1, IP3R, CAMKKII and CaM in the hippocampus of VaD rats. Quantitative analysis of (B) PLCγ1, (C) CAMKKII, (D) p-PLCγ1, (E) IP3R and (G) CaM expression. The densities of the bands were normalized with respect to the values of GADPH and β-actin. Values were expressed as means ± SD ( n = 3). ### P < 0.001 versus sham-surgery group. ** P < 0.01, *** P < 0.001 versus model group.

Article Snippet: Following washes and blocking (5% skim milk, 1 h), membranes were probed overnight (4°C) with primary antibodies against p-PLCγ1 (1:2,000 dilution, AF3210, AB_2834502, Affinity Biosciences, United States), PLCγ1 (1:2,000 dilution, AF6210, AB_2835091, Affinity Biosciences, United States), IP3R (1:2000 dilution, DF3000, AB_2840979, Affinity Biosciences, United States), CAMKKII (1:1,000 dilution, DF4793, AB_2837147, Affinity Biosciences, United States), GAPDH (1:50,000 dilution, 60004-1-Ig, AB_2107436, Proteintech, China), CaM (1:2,000 dilution, AF6353, AB_2835158, Affinity Biosciences, United States) and β-actin (1:10,000 dilution, AF7018, AB_2839420, Affinity Biosciences, United States).

Techniques: Expressing, Western Blot

Effects of TQHS granules on OGD/R-induced cytotoxicity SH-SY5Y cells and the mRNA expressions of PLCγ1, IP3R, and TrkB. (A) Effects of U73122 on normal SH-SY5Y cell viability. (B,C) Effects of B-U73122 and (C) TQHS granules on OGD/G cell viability. (D–F) RT-qPCR analyzed (D) TrkB, (E) IP3R and (F) PLCγ1 mRNA expression levels. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus control group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus OGD/R group.

Journal: Frontiers in Aging Neuroscience

Article Title: Exploring the neuroprotective mechanism of Tongqiao Huashuan granules in vascular dementia based on PLCγ1/IP3R signaling pathway

doi: 10.3389/fnagi.2026.1719664

Figure Lengend Snippet: Effects of TQHS granules on OGD/R-induced cytotoxicity SH-SY5Y cells and the mRNA expressions of PLCγ1, IP3R, and TrkB. (A) Effects of U73122 on normal SH-SY5Y cell viability. (B,C) Effects of B-U73122 and (C) TQHS granules on OGD/G cell viability. (D–F) RT-qPCR analyzed (D) TrkB, (E) IP3R and (F) PLCγ1 mRNA expression levels. Values were expressed as means ± SD ( n = 3). ## P < 0.01, ### P < 0.001 versus control group. * P < 0.05, ** P < 0.01, *** P < 0.001 versus OGD/R group.

Article Snippet: Following washes and blocking (5% skim milk, 1 h), membranes were probed overnight (4°C) with primary antibodies against p-PLCγ1 (1:2,000 dilution, AF3210, AB_2834502, Affinity Biosciences, United States), PLCγ1 (1:2,000 dilution, AF6210, AB_2835091, Affinity Biosciences, United States), IP3R (1:2000 dilution, DF3000, AB_2840979, Affinity Biosciences, United States), CAMKKII (1:1,000 dilution, DF4793, AB_2837147, Affinity Biosciences, United States), GAPDH (1:50,000 dilution, 60004-1-Ig, AB_2107436, Proteintech, China), CaM (1:2,000 dilution, AF6353, AB_2835158, Affinity Biosciences, United States) and β-actin (1:10,000 dilution, AF7018, AB_2839420, Affinity Biosciences, United States).

Techniques: Quantitative RT-PCR, Expressing, Control

Potential mechanism underlying the neuroprotective effects of TQHS granules in VaD rats and SH-SY5Y cells. TQHS granules improved cognitive function in VaD rats, generated IP3 through PLCγ1-mediated catalytic hydrolysis of PIP2, and then affected the expression of IP3R downstream of the pathway, thus protecting the brain from VaD-induced oxidative stress and calcium overload, and further induced antioxidant activity and regulated intracellular calcium overload mechanisms.

Journal: Frontiers in Aging Neuroscience

Article Title: Exploring the neuroprotective mechanism of Tongqiao Huashuan granules in vascular dementia based on PLCγ1/IP3R signaling pathway

doi: 10.3389/fnagi.2026.1719664

Figure Lengend Snippet: Potential mechanism underlying the neuroprotective effects of TQHS granules in VaD rats and SH-SY5Y cells. TQHS granules improved cognitive function in VaD rats, generated IP3 through PLCγ1-mediated catalytic hydrolysis of PIP2, and then affected the expression of IP3R downstream of the pathway, thus protecting the brain from VaD-induced oxidative stress and calcium overload, and further induced antioxidant activity and regulated intracellular calcium overload mechanisms.

Article Snippet: Following washes and blocking (5% skim milk, 1 h), membranes were probed overnight (4°C) with primary antibodies against p-PLCγ1 (1:2,000 dilution, AF3210, AB_2834502, Affinity Biosciences, United States), PLCγ1 (1:2,000 dilution, AF6210, AB_2835091, Affinity Biosciences, United States), IP3R (1:2000 dilution, DF3000, AB_2840979, Affinity Biosciences, United States), CAMKKII (1:1,000 dilution, DF4793, AB_2837147, Affinity Biosciences, United States), GAPDH (1:50,000 dilution, 60004-1-Ig, AB_2107436, Proteintech, China), CaM (1:2,000 dilution, AF6353, AB_2835158, Affinity Biosciences, United States) and β-actin (1:10,000 dilution, AF7018, AB_2839420, Affinity Biosciences, United States).

Techniques: Generated, Expressing, Antioxidant Activity Assay